PrintProteomics Core
The Proteomics Core Facility is located in room 2219 of the new Bioscience Research Building. The facility is equipped with
a ThermoFinnigan LTQ Orbitrap XL mass spectrometer, and an Accela HPLC with the
ability to run at ultra high pressure for high throughput analysis. A nano LC
system is purchased and will be interfaced to the LTQ Orbitrap to enable optimum
sensitivity for handling low level samples. The facility also maintains 2
separate data stations dedicated for proteomics data processing, database
searching, and generation of reports. Several popular software packages are
installed on the data station, including Bioworks, Mascot server, Mascot
Distiller, Scaffold Distiller, Seive, Proteome Discoverer, and Prosight PC. For
protein separation, the Facility maintains an Agilent
3100 offgel fractionator that performs isoelectric focusing based
fractionation in solution.
The Proteomics Core Facility is a fee for service facility supported by the College of Chemical and Life Sciences. The facility offers service to all researchers in the region, with priority given to users from the College of Chemical and Life Sciences. Routine service includes molecular weight determination of intact proteins by LCMS and protein identification through tryptic digestion, LCMSMS analysis and database searching. Protein samples can be submitted as lyophilized powders, solutions, or gel slices. Please refer to the “How to prepare your samples” document for detailed information.
The facility also welcomes collaborative research on projects beyond routine services. Please contact the facility director Dr. Yan Wang to initiate a collaborative research.
As a start up, the facility is currently offering a 40% discount on service charges
Contact
Dr. Yan Wang
2219 Bioscience Research Building
College Park, MD
20742
301-405-6764 (office)
301-405-8425 (lab)
Email: yanwang@umd.edu
